Protocols
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Activity Test - Procedure for immunoprecipitation of native chromatin proteins
AMPure® XP Size Selection - IP-Star Compact
AMPure® XP Size Selection - IP-Star® Compact -
Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL
CATS RNA-seq v2 library preparation protocol on RNA from FFPE-samples
ChIP - Just 4 steps to foolproofing fragmentation for ChIP using Bioruptor® Standard, Plus or Pico
ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus
ChIP from plant material with the LowCell# ChIP Kit
ChIP from tissues with the LowCell# ChIP kit
ChIPettor Protocols
Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico
Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus
Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml
Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
CRISPR/Cas9 editing: mutation detection with mismatch cleavage assay
Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
DNA Shearing for Bioruptor® Pico (2013-2019) - 50 μl Volume
DNA Shearing for Bioruptor® Pico (2013-2019) - Standard 100 μl Volume
DNA Shearing for Bioruptor® Standard and Plus
DNA Shearing for Bioruptor® XL
DNA shearing for Next-Generation Sequencing with the Bioruptor® Pico (2013-2019), 0.2 ml Bioruptor® microtubes and the corresponding holder
DNA Shearing with Bioruptor® Pico (2013-2019) - Small Volume (10 µl)
Dot blot protocol for 5-hydroxymethylcytosine monoclonal rat antibody
FFPE DNA extraction protocol using the Bioruptor®
iDeal Library Preparation kit - IP-Star® compact
iST Sample Preparation with the Bioruptor®
Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
Mass-Spectrometry: procedure for shotgun proteomics
MicroPlex Library Preparation kit™ v2 - IP-Star Compact
NEBNext® ChIP-Seq Library Prep for Illumina - IP-Star Compact
NEBNext® ChIP-Seq Library Prep for Illumina - IP-Star® Compact -
PCR amplicons shearing using the Bioruptor Pico
Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus
Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico
RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit
RNA Shearing for Bioruptor® NGS and Pico
TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
Transfection of CRISPR/Cas9 Nuclease NLS ribonucleoprotein (RNP) into adherent mammalian cells using Lipofectamine® RNAiMAX
TruSeq DNA sample preparation - IP-Star® Compact
TruSeqTM ChIP Sample Preparation - IP-Star® Compact -
TruSeq™ ChIP Sample Preparation - IP-Star® Compact
TruSeq™ DNA PCR-Free Sample Preparation - IP-Star Compact
TruSeq™ RNA Sample Preparation - IP-Star Compact
TruSeq™ Stranded RNA Sample Preparation - IP-Star® Compact
Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation
Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing