ZHX2 polyclonal antibody - Classic

Figure 1. ChIP results obtained with the Diagenode antibody directed against ZHX2
ChIP assays were performed using HepG2 cells, the Diagenode antibody against ZHX2 (Cat. No. C15410260) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the CDC25C and AFTPH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ZHX2
ChIP was performed on sheared chromatin from 5 million HepG2 cells using 5 μg of the Diagenode antibody against ZHX2 (Cat. No. C15410260). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of chromosomes 1 (fig 2A and B) and in two genomic regions surrounding the CDC25C and AFTPH genes on chromosome 5 and 2, respectively (fig 2C and D).

Figure 3. Western blot analysis using the Diagenode antibody directed against ZHX2
Whole cell extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against ZHX2 (Cat. No. C15410260) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 4. Immunofluorescence with the Diagenode antibody directed against ZHX2
HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against ZHX2 (Cat. C15410260) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.

Figure 5. Immunohistochemistry using the Diagenode antibody directed against ZHX2
Formalin fixed paraffin embedded SAS xenograft tissue was stained with the Diagenode antibody against ZHX2 (Cat. No. C15410260) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.