THOC1 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against THOC (nuclear matrix Protein p84 (THO complex 1), using a recombinant protein.
| Lot | 40163 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| ICC/IF | 1:100 - 1:1,000 | Fig 3 |
| IHC | 1:100 - 1:1,000 | Fig 4 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 5 |
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. THOC1 ChIP results
ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or THOC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to calreticulin.
Figure 2. THOC1 IP results
THOC1 immunoprecipitates THOC1 (p84) protein in IP experiments. IP Sample: HepG2 whole cell extract A: 30 μg whole cell extract of THOC1 protein expressing HepG2 cells B: Control with 3 μg of pre-immune rabbit IgG C: Immunoprecipitation of THOC1 by 3 μg of THOC1 antibody. The immunoprecipitated THOC1 protein was detected by western blot with the THOC1 antibody diluted 1:1,000.
Figure 3. THOC1 IF results
THOC1 antibody detects THOC1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: THOC1 protein stained by THOC1 antibody diluted 1:500. Blue: Hoechst 33342 staining.
Figure 4. THOC1 IHC results
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using THOC1 antibody at a 1:500 dilution.
Figure 5. THOC1 Western blot results
Sample: 30 μg of A549 whole cell lysate 7.5% SDS PAGE THOC1 antibody diluted 1:1,000
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet THOC1 C15410243 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
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