TFIIF Antibody
Polyclonal antibody raised in rabbit against TFIIF (general transcription factor IIF, polypeptide 1, 74kDa), using a recombinant protein.
| Lot | 40723 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3, 4 |
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B01060010Bioruptor® Pico sonication device
Validation Data
Figure 1. TFIIF ChIP results
ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
Figure 2. TFIIF IP results
TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of TFIIF protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C: Immunoprecipitation of TFIIF with 2.5 μg of TFIIF antibody (C15410256) The immunoprecipitated TFIIF protein was detected by western blot with the TFIIF antibody diluted 1:1,000.
Figure 3. Western blot
Sample: 30 μg of whole cell lysate A: 293T B: A431 C: HeLa 7.5% SDS PAGE TFIIF antibody dilution 1:3,000.
Figure 4. Western blot
Sample: 30 μg of NIH-3T3 whole cell lysate 7.5% SDS PAGE TFIIF antibody dilution 1:3,000
Applications
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet TFIIF C15410256 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
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