SMAD3 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against SMAD3 (SMAD family member 3), using a recombinant protein.
| Lot | 40044 |
|---|---|
| Concentration | 0.78 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| ICC/IF | 1:100 - 1:1,000 | Fig 3 |
| IHC | 1:500 | Fig 5 |
| Immunoprecipitation | 1:500 - 1:1,000 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 4 |
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. ChIP
SMAD3 antibody immunoprecipitates SMAD3 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD3 antibody (C15410252) The precipitated DNA was detected by PCR with primer set targeting to SMAD7 promoter.
Figure 2. Immunoprecipitation
SMAD3 antibody immunoprecipitates SMAD3 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell extract A. 50 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD3 protein by 2 μg of SMAD3 antibody (C15410252). The immunoprecipitated SMAD3 protein was detected by western blot with the SMAD3 antibody (C15410252) diluted 1:1,000.
Figure 3. Immunofluorescence
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using SMAD3 (C15410252) antibody at a 1:200 dilution.
Figure 4. Western Blot
Sample: 30 μg of Jurkat whole cell lysate 7.5% SDS PAGE C15410252 diluted 1:5,000
Figure 5. Immunohistochemistry
SMAD3 antibody detects SMAD3 protein at cytosol and nucleus on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded gastric carcinoma. SMAD3 antibody (C15410252) dilution: 1:500.
Applications
| ELISA Enzyme-linked immunosorbent assay. Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet SMAD3 C15410252 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
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