Polyclonal antibody raised in rabbit against SETDB1 (SET domain, bifurcated 1), using a recombinant protein.
| Lot | 40471 |
|---|---|
| Concentration | 0.63 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunoprecipitation1:100 - 1:500Fig 3
| Applications | Suggested dilution * | References |
|---|---|---|
| Western Blotting | 1:500 - 1:3,000 | Fig 1 |
| Immunoprecipitation | 1-100 - 1:500 | Fig 2 |
* Optimal dilutions/concentrations should be determined by the researcher.
Figure 1. Western Blot analysis using the Diagenode antibody directed against SETDB1
Whole cell extracts (30 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody against SETDB1 (Cat. No. C15410249) diluted 1:10,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 2. Immunoprecipitation using the Diagenode antibody directed against SETDB1
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 4 μg of the Diagenode antibody against SETDB1 (Cat. No. C15410249, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated SETDB1 protein was detected by western blot with the SETDB1 antibody diluted 1:1,000.
| Datasheet SETDB1 C15410249 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
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