SATB1 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against human SATB1, using a recombinant protein.
| Lot | 40499 |
|---|---|
| Concentration | 0.81 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| IHC | 1:100 - 1:1,000 | Fig 4 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3, 5 |
-
B01060010Bioruptor® Pico sonication device
-
B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. ChIP
ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.
Figure 2. Immunoprecipitation
SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell extract. A. Control with 2 μg of preimmune rabbit IgG (Cat. No. C15410206). B. Immunoprecipitation of SATB1 protein by 2 μg of SATB1 antibody 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody diluted 1:1,000.
Figure 3. Western Blot
SATB1 antibody detects SATB1 protein by western blot analysis. Jurkat whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (C15410248) diluted 1:500
Figure 4. Immunohistochemistry
Immunohistochemical analysis of paraffin-embedded Colon, using the SATB1 antibody (C15410248) at a 1:250 dilution.
Figure 5. Western Blot
SATB1 antibody detects SATB1 protein by western blot analysis. THP-1 whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (C15410248) diluted 1:500.
Applications
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet SATB1 C15410248 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: SATB1 polyclonal antibody - Classic (Diagenode Cat# C15410248-100 Lot# 40499). Click here to copy to clipboard. Using our products in your publication? Let us know! |