Total RNA-seq Service
Exploring the whole transcriptome allows to gain insight of potential transcriptional and regulatory network mechanisms. With Total RNA-seq services, we will sequence the whole transcriptome and record the complete set of RNA transcripts, including mRNAs and long non-coding RNAs, present in a biological sample under specific circumstances. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.
Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation can be performed with an optional rRNA depletion step to enable cost-effective sequencing of RNA samples.
High discovery power with total RNA-seq
- Detection of both coding and non-coding RNAs with optional rRNA depletion
- Recognition of long coding RNAs involved in chromatin remodeling and transcriptional/post-transcriptional regulation
- Investigation of the structure of genes, their splicing patterns and other post transcriptional modifications
- Suitable for a broad range of input amounts
- Compatible with FFPE samples
- Fast turnaround time (from 5 to 6 weeks)
- Project size: minimum of 24 samples
- Sample type: total isolated RNA
- Required quantities: minimum 5 ng of total RNA (Human, Mouse, Rat) or 100 ng (Plant)
- Shipment conditions: shipped in 1.5 mL or 0.5 mL tubes on dry ice
- RNA quality control
- Determination of RIN value (RNA integration number)
- Measurement of RNA concentration
- Library preparation
- Optional rRNA depletion
- Stranded library construction
- Quality control of libraries prior to sequencing
- Sequencing on Illumina platforms
- 50 million raw reads per sample
- Data analysis (optional for additional fee)
- Standard bioinformatic analysis
- Advanced bioinformatic analysis
- Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
- Demultiplexing, read quality control, trimming and filtering
- Alignment to reference genome
- Quantification of coding and non-coding RNA biotypes with specialized quantification tool MGCount (software developed by Diagenode)
- Report with sequencing statistics
- Raw data in FASTQ format
- FastQC report
- Alignment in BAM format
- Un-normalized read counts
- Differential expression analysis
- Gene ontology terms analysis
- Pathway analysis
- Alternative splicing analysis
- Novel transcript identification
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G02100000Bioinformatics Data Mining Service
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G02120000
mRNA-seq Service
Sample Requirements
Services Workflow
What our service includes
Bioinformatics Analysis
Standard bioinformatic analysis
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Advanced bioinformatic analysis
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Provided files: |
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Documents
| The Diagenode Epigenetics custom service POSTER Complete workflows for genome-scale DNA methylation and histone marks analysis Epigenetics is cr... | Download |
Publications
How to properly cite our product/service in your workWe strongly recommend using this: Total RNA-seq Service (Hologic Diagenode Cat# G02030000). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Long-term effects of myo-inositol on traumatic brain injury: Epigenomic and transcriptomic studies |
Inflammatory stress-mediated chromatin changes underlie dysfunction in endothelial cells |
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