Recombinant mouse TDG
Active full length recombinant mouse N-terminal His tagged TDG (Thymine-DNA Glycosylase) protein, produced in SF9 insect cells.
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Product Information
TDG recombinant protein
Other names
HTDG
Target description
TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine.
Concentration
0.5µg/µl
Specificity
Mouse
Purity
Purified using FPLC, >95% purity as determined by SDS-PAGE.
Storage Buffer
20mM Tris-Cl pH 7.6, 1 mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5 mM PMSF and 1 mM DTT.
Storage
Store at-80°C; guaranteed stable for 2 years from date of receipt when stored properly.
Precautions: This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Quality Control
Figure 1.
SDS page of the TDG recombinant protein. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Testimonials
The Diagenode TDG recombinant protein (Cat. No. C23020101) is a very good tool. I used it as positive control in western blot analysis and during TDG ELISA activity assays. The protein is really active! Indeed the TDG recombinant protein perfectly works in glycosylase activity assays using marked oligos.
Dr Francesco Spallotta, Division of Cardiovascular Epigenetics, Goethe University, Frankfurt am Main, Germany
Documents
| TDG recombinant protein DATASHEET Datasheet of TDG recombinant protein | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: Recombinant mouse TDG (Diagenode Cat# C23020101). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Stable Oxidative Cytosine Modifications Accumulate in Cardiac Mesenchymal Cells From Type2 Diabetes Patients: Rescue by α-Ketoglutarate and TET-TDG Functional Reactivation |