PRMT7 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against PRMT7 (protein arginine methyltransferase 7), using a recombinant protein.
| Lot | 40674 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| ICC/IF | 1:100 - 1:1,000 | Fig 3 |
| IHC | 1:100 - 1:1,000 | Fig 5, 6, 7 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 4 |
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. ChIP
ChIP was performed with NIH3T3 chromatin extract and 5 μg of either control rabbit IgG or PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.
Figure 2. Immunoprecipitation
PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 μg of PRMT7 antibody (Cat. No. C15410247) The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody (C15410247) diluted 1:1,000.
Figure 3. Immunofluorescent analysis
PRMT7 antibody detects PRMT7 protein in the cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody (Cat. No. C15410247) diluted 1:500. Blue: Hoechst 33342 staining.
Figure 4. Western blot Sample: 30 μg of PC-3 whole cell lysate 7.5% SDS PAGE PMRT7 antibody diluted 1:2,000
Figure 5. Immunohistochemical analysis
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PRMT7 (C15410247) antibody at a 1:500 dilution.
Figure 6. Immunohistochemical analysis
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7 (C15410247) antibody at a 1:500 dilution.
Figure 7. Immunohistochemical analysis
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PRMT7 (C15410247) antibody at a 1:500 dilution.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet PRMT7 C15410247 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: PRMT7 polyclonal antibody - Classic (Diagenode Cat# C15410247-100 Lot# 40674). Click here to copy to clipboard. Using our products in your publication? Let us know! |