PKNOX1 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against PKNOX1 (PBX/knotted 1 homeobox 1), using a recombinant protein.
| Lot | 40870 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| Immunofluorescence | 1:100 - 1:1,000 | Fig 1 |
| Immunoprecipitation | 1:100 - 1:1,000 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3 |
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. PKNOX1 IF results
PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 antibody diluted 1:1,000. Blue: Hoechst 33342 staining.
Figure 2. PKNOX1 Immunoprecipitation results
PKNOX1 antibody immunoprecipitates PKNOX1 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 μg of PKNOX1 antibody The immunoprecipitated PKNOX1 protein was detected by western blot with the PKNOX1 antibody diluted 1:1,000.
Figure 3. PKNOX1 Western blot results
Sample: 30 μg of whole cell lysate A: HepG2 B: HepG2 nucleus 10% SDS PAGE PKNOX1 antibody diluted 1:2,000.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet PKNOX1 C15410267 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: PKNOX1 polyclonal antibody - Classic (Diagenode Cat# C15410267-100 Lot# 40870). Click here to copy to clipboard. Using our products in your publication? Let us know! |