PARP1 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against PARP1 (poly (ADP-ribose) polymerase 1), using a recombinant protein.
| Lot | 42914 |
|---|---|
| Concentration | 0.17 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 µg/ChIP | Fig 1 |
| ICC/IF | 1:100 - 1:1,000 | Fig 3 |
| IHC | 1:100 - 1:1,000 | Fig 4 |
| Immunoprecipitation | 1:100-1:500 | Fig 6 |
| Western Blotting | 1:5,000 - 1:20,000 | Fig 2, 5 |
* Optimal dilutions/concentrations should be determined by the researcher.
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. ChIP
ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
Figure 2. Western Blot
PARP1 antibody detects PARP1 protein by Western blot analysis. A. 30 μg HCT116 whole cell extract (untreated) B. 30 μg HCT116 whole cell extract (30 μM cisplatin treatment for 24hr) 7.5 % SDS-PAGE PARP1 antibody (Cat. No. C15410245) dilution: 1:5,000
Figure 3. Immunofluorescent analysis
PARP1 antibody detects PARP1 protein in the nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 protein stained by PARP1 antibody (Cat. No. C15410245) diluted 1:500. Blue: Hoechst 33342 staining.
Figure 4. Immunohistochemical analysis
PARP1 antibody detects PARP1 protein in the nucleus of HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody (Cat. No. C15410245) dilution: 1:500.
Figure 5. Western Blot
Sample: 30 μg of whole cell lysate A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE C15410245 dilution 1:10,000
Figure 6. Immunoprecipitation
PARP1 antibody immunoprecipitates PARP1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 30 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PARP1 protein with 4 μg PARP1 antibody (Cat. No. C15410245) 5 % SDS-PAGE The immunoprecipitated PARP1 protein was detected by PARP1 antibody (C15410245) diluted 1:500.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| PARP1 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against PARP1 (poly (ADP-ribose) polymerase 1), using a reco... | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: PARP1 polyclonal antibody - Classic (Diagenode Cat# C15410245-100 Lot# 42914). Click here to copy to clipboard. Using our products in your publication? Let us know! |