NRF2 polyclonal antibody
Other names: NFE2L2, HEBP1, TFIIE-B, FE
Polyclonal antibody raised in rabbit against NRF2 (NF-E2-Related Factor 2), using a recombinant protein.
| Lot | 42347 |
|---|---|
| Concentration | 0.58 μg/μl |
| Species reactivity | Human, rat |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody. |
|---|---|
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 20% glycerol and 0.025% ProClin 300. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 5 μg/ChIP | Fig 1 |
| Immunofluorescence | 1:500 | Fig 2 |
| Immunohistochemistry | 1:500 | Fig 3 |
| Western Blotting | 1:1,000 | Fig 4, 5 |
| Immunoprecipitation | 5 μg/IP | Fig 6 |
* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 µg per IP.
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. ChIP results obtained with the Diagenode antibody directed against NRF2
ChIP was performed on HeLa cells with 5 µg of the Diagenode antibody against NRF2 (Cat. No. C15410242). IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the GCLC gene. Figure 1 shows the fold enrichment over the IgG negative control.
Figure 2. Immunofluorescence using the Diagenode antibody directed against NRF2
HeLa cells were stained with the Diagenode antibody against NRF2 (Cat. No. C15410242). Cells were fixed with 4% formaldehyde for 15 min at RT and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the NRF2 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Phalloidin, a cytoskeleton marker.
Figure 3. Immunohistochemistry using the Diagenode antibody directed against NRF2
Paraffin-embedded Cal27 xenograft was analysed by immunohistochemical analysis, using the Diagenode antibody against NRF2 (Cat. No. C15410242) diluted 1:500.
Figure 4. Western blot analysis using the Diagenode antibody directed against NRF2
Whole cell extracts from 293T cells (30 µg) transfected with a 3xFlag-tagged NRF2 expression vector (lane 2) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against NRF2 (Cat. No. C15410242) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 5. Western blot analysis using the Diagenode antibody directed against NRF2
Nuclear extracts from MDA-MB-231 cells (30 µg) treated with 30 µM tBHQ for 4 hours (lane 2) or untreated control cells (lane 1) were analysed by western blot using the Diagenode antibody against NRF2 (cat. No. C15410242) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 6. Immunoprecipitation using the Diagenode antibody directed against NRF2
Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 5 µg of the Diagenode antibody against NRF2 (Cat. No. C15410242) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated NRF22 protein was detected by western blot with the NRF2 antibody diluted 1:1,000.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
| IP Immunoprecipitation Read more |
Documents
| Datasheet NRF2 C15410242 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: NRF2 polyclonal antibody (Diagenode Cat# C15410242-100 Lot# 42347). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Suppression of AMPK/aak-2 by NRF2/SKN-1 down-regulates autophagy during prolonged oxidative stress. |