MECP2 monoclonal antibody - Classic
Monoclonal antibody raised in mouse against MeCP2 (Methyl-CpG-binding domain protein 2), using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse |
| Type | Monoclonal |
| Purity | Affinity purified |
|---|---|
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 | Fig 1 |
| Immunofluorescence | 1:400 | Fig 2 |
Validation data
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against MeCP2
Whole cell extracts from HeLa (lane 1) or MEF (lane 2) cells were analysed by Western blot using the Diagenode antibody against MeCP2 (cat. No. C15200225) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against MeCP2
HeLa cells were stained with the Diagenode antibody against MeCP2 (cat. No. C15200225) diluted 1:400 followed by an anti-mouse antibody conjugated to Alexa488 (upper figure). The bottom figure shows staining of the nuclei with DAPI.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
Documents
| Datasheet MECP2 monoclonal DATASHEET Datasheet of MECP2 monoclonal antibody | Download |
Publications
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