MCM6 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against human MCM6, using a recombinant protein.
| Lot | 41521 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/IP | Fig 1 |
| ICC/IF | 1:100 - 1:1,000 | Fig 5 |
| Immunohistochemistry | 1:100 - 1:1,000 | Fig 3, 4 |
| Immunoprecipitation | 1:100 - 1:500 | Fig 6 |
| Western Blotting | 1:500 - 1:3,000 | Fig 2 |
-
B01060010Bioruptor® Pico sonication device
-
B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. MCM6 ChIP results
ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or MCM6 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
Figure 2. MCM6 Western blot results
MCM6 antibody detects MCM6 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 7.5 % SDS-PAGE MCM6 antibody dilution: 1:1,000
Figure 3. MCM6 IHC results
MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MCM6 antibody dilution: 1:500.
Figure 4. MCM6 IHC results
MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. MCM6 antibody dilution: 1:500.
Figure 5. MCM6 IF results
MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM6 protein stained by MCM6 antibody diluted 1:500. Blue: Hoechst 33342 staining.
Figure 6. MCM6 IP results
MCM6 antibody immunoprecipitates MCM6 protein in IP experiments. IP Sample: 293T whole cell extract A : 30 μg whole cell extract of MCM6 protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of MCM6 by 3 μg of MCM6 antibody. The immunoprecipitated MCM6 protein was detected by western blot using the MCM6 antibody diluted 1:1,000.
Applications
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
Documents
| Datasheet MCM6 C15410239 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Publications
How to properly cite this product in your workDiagenode strongly recommends using this: MCM6 polyclonal antibody - Classic (Diagenode Cat# C15410239-100 Lot# 41521). Click here to copy to clipboard. Using our products in your publication? Let us know! |