L3MBTL2 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against L3MBTL2 (l(3)mbt-like 2 (Drosophila)), using a recombinant protein.
| Lot | 40009 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
|---|---|
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP assay | 5 μg/ChIP | Fig 1 |
| Immunoprecipitation | 1:100 - 1:1,000 | Fig 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3 |
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B01060010Bioruptor® Pico sonication device
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B03000002IP-Star® Compact Automated System
Validation Data
Figure 1. L3MBTL2 ChIP results
L3MBTL2 antibody immunoprecipitates L3MBTL2 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of L3MBTL2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.
Figure 2. L3MBTL2 IP results
L3MBTL2 antibody immunoprecipitates L3MBTL2 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of L3MBTL2 protein by 2.5 μg of L3MBTL2 antibody. The immunoprecipitated L3MBTL2 protein was detected by western blot with the L3MBTL2 antibody diluted at a 1:1,000.
Figure 3. L3MBTL2 Western blot results
Sample: 30 μg MOLT4 whole cell lysate 7.5% SDS PAGE L3MBTL2 antibody diluted 1:1,000
Applications
Documents
| Datasheet L3MBTL2 C15410238 DATASHEET Datasheet description | Download |
Publications
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