JMJD6 Antibody

Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD6
ChIP assays were performed using HeLa cells, the Diagenode antibody against JMJD6 (Cat. No. C15410318) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the SPI1 and SPOCD1, used as positive controls, and for the HBB promoter and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD6
Whole protein extracts from mouse heart tissue were analysed by Western blot using the Diagenode antibody against JMJD6 (Cat. No. C15410318). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 3. Immunohistochemistry using the Diagenode antibody directed against JMJD6
Formalin fixed paraffin embedded mouse brain tissue was stained with the Diagenode antibody against JMJD6 (Cat. No. C15410318) diluted 1:200 followed by a peroxidase labelled goat anti-rabbit secondary antibody.