Hydroxymethyl dCTP

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Catalog Number

Format

Order

C11000002
(AF-102-0300)

250 µl

DISCONTINUED

Hydroxymethyl dCTP can be used as a substrate for MethylTaq DNA polymerase (Cat. No. AF-103-0250) permitting the amplification of DNA containing hydroxymethylated cytosine in place of cytosine.

Characteristics

Full name: 5-hydroxymethyl-2'-deoxycytidine-5'-triphosphate (Lithium Salt)

Formula: C₁₀H₁₄Li₄N₃O₁₄P₃

Molecular Weight: 520.9 g/mol

Lambda max: 275 nm

Description

hmdCTP is supplied as 100 mM aqueous solution titrated to pH 7.5
> 99% pure by spectral and HPLC analysis
hmdCTP can be enzymatically incorporated into DNA with MethylTaq DNA polymerase (Cat. No. AF-103-0250)
Hyrdoxymethylated substrates can be ligated by standard ligases
Free of endo-, exodeoxyribonuclease, phosphatise and nicking activities

Documents

Datasheet Hydroxymethyl dCTP AF-102 DATASHEET
Datasheet description

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Publications

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