Huntingtin monoclonal antibody - Classic
Monoclonal antibody raised in mouse against Huntigntin, using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse |
| Type | Monoclonal |
| Purity | Affinity purified |
|---|---|
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 | Fig 1 |
| Immunofluorescence | 1:400 | Fig 2 |
Validation data
Western blot analysis using the Diagenode monoclonal antibody directed against Huntingtin
Mouse brain lysates were analysed by Western blot using the Diagenode antibody against Huntingtin (Cat. No. C15200226) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left
Immunofluorescence using the Diagenode monoclonal antibody directed against Huntingtin
SKNSH cells were stained with the Diagenode antibody against Huntingtin (Cat. No. C15200226) diluted 1:400 followed by an anti-mouse antibody conjugated to DyLight 488 (green color). WGA conjugated to Alexa Fluor 594 was used to stain the plasma membranes (red). The nuclei were stained with DAPI (blue).
Background
Huntingtin (HTT, UniProt/Swiss-Prot entry P42858) may play a role in microtubule-mediated transport or vesicle function and is required for normal development. It is widely expressed as 2 alternatively polyadenylated transcripts, the larger one being predominantly present in adult and fetal brain. The HTT gene is linked to Huntington’s disease, a neurodegenerative disorder characterized by loss of striatal neurons, which is thought to be caused by an expanded, unstable trinucleotide repeat. Whether this leads to loss of function or gain of function is not clear. The expanded repeat may increase the affinity of the HTT protein for the huntingtin-associated protein-1, which is highly expressed in brain and seems to inhibit HTT expression.
Applications
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
Documents
| Datasheet C15200226 Huntingtin monoclonal antibody DATASHEET Huntingtin monoclonal antibody - Classic | Download |
Publications
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