Human Recombinant His- and FLAG-tagged full-length DNMT1

Full lenght human DNMT1 recombinant protein

Target description

DNA methylation is recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. DNA (cytosine-5)-methyltransferase1 (DNMT1) preferentially methylates hemi-methylated DNA, in a CpG context, to maintain the methylation pattern in the new strand of replicated DNA.

Full-length human recombinant DNMT1 (residues 2-1616 (C-terminus); Genbank Accession # NM_001379) expressed in Sf9 insect cells with an N-terminal His-tag and a C-terminal Flag-Tag. The molecular weight of the protein, including the His-tag and Flag-tag is 182 kDa.

TExpression system: Baculovirus/ Sf9 insect cells

Concentration

25µg at 0,5µg /µl

Specificity 

Human

Application 

Recombinant DNMT1 is suitable for protein-protein interaction studies, DNA methylation assays, drug screening, structural studies, etc. 

Storage Buffer

25 μg recombinant DNMT1 protein supplied at a concentration of 0.5 μg/μl in 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 100mM EDTA pH 8, 0.01% NP-40, and 10% glycerol.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Precautions: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Sequence:

      MHHHHHHLEPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKE                   KLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNRE                   VNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRK                  STRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLP                   AEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDE                 DGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTE                KKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDAN                ESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLE               GGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYI      SKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDE                 AGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLV                   YQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRS                   KQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKT                  DGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFC                 AGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLE               GDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQL                EDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEH                   YRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPEN THKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGP                   NRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEI KLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFT EDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSK FKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTF GVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKF VSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQ YQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYT HHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAG LYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLF GNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKDAAAD                                                                 YKDDDDK*