H3T6pK9me3 Antibody
Polyclonal antibody raised in rabbit against Histone H3 (p Thr6), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.55 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP | 2-5 μg/million cells | |
| Immunohistochemistry | 1:200 | |
| Immunofluorescence | 1:100 | Fig 1 |
| Western Blotting | 1:500 | Fig 2, 3 |
| Dot Blotting | 1:1,000 | Fig 4 |
Validation Data
Figure 1. H3T6pK9me3 antibody ChIP results
Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin.
Figure 2 H3T6pK9me3 antibody Immunofluorescence results
Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue).
Figure 3. H3T6pK9me3 antibody Western blot results
Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 4. H3T6pK9me3 antibody Western blot results
Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 5. H3T6pK9me3 antibody Dot blot results
Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Target Description
Phosphorylation as a post-translational modification on histone H3 modulates the ability of proteins to recognize and bind to the H3 tail. When additional modifications to H3 are present, in addition to the native T6 phosphorylation, there is an amplified effect. Several authors suggest that the complexity of these combined PTMs on histone tails should be described as a convoluted language, rather than as a strict code. Since the H3T6 phosphorylation seems to be constitutively present, its de-phosphorylation could be highly significant and affect transcription, repair and replication.
Applications
| IHC Immunohistochemistry Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| DB Dot blotting Read more |
| ChIP-qPCR (ab) Read more |
Documents
| Datasheet H3T6p C15410282 DATASHEET Datasheet description | Download |
Publications
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