Protein Analysis

Protein extraction

Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Diagenode's Bioruptor^® deliver high quality protein.

Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).

Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).

Recommended products for Protein extraction

Cat. No.	Product	Format	Order
B01080010	Bioruptor^® Pico sonication device The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing sam...	1 unit

B01020014 Bioruptor^® Plus sonication device
// The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling... 1 unit

C30010010-50 1.5 ml Bioruptor® Plus TPX microtubes
TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultra... 50 pc B01200045 iST cartridge holder for the Bioruptor Pico (PreOmics)
The iST cartridge holder has been especially designed for being used with the Bioruptor® Pico sonication device and with the PreOmics iST sample preparat... 1 pack C20000021 Protein Extraction beads
Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein... 20 g C20000020 Protein Extraction kit for Bioruptor^® Plus
Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein... 50 rxns B01201140 Tube holder for 1.5 ml tubes - Bioruptor^® Pico
The tube holder for 1.5 ml tubes has been especially designed for being used with the Bioruptor® Pico and to guarantee homogeneity of chromatin or DNA sh... 1 unit

Related protocols

Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...	Download
TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor® The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein com...	Download
Activity Test - Procedure for immunoprecipitation of native chromatin proteins Proteins play role in almost all cellular processes, and the study of their activity is crucial f...	Download
Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor® The regular protocol for the extraction of histone requires an acid extraction, making impossible...	Download
Mass-Spectrometry: procedure for shotgun proteomics Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Sh...	Download
Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck ...	Download
iST Sample Preparation with the Bioruptor® The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e...	Download