Chromatin Analysis

ChIP-seq

Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.

The ChIP-seq workflow

Chromatin preparation: Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.
Chromatin shearing: Fragment chromatin by sonication to desired fragment size (100-500 bp)
Chromatin IP: Capture protein-DNA complexes with specific ChIP-seq grade antibodies against the histone or transcription factor of interest
DNA purification: Reverse cross-links, elute, and purify
NGS Library Preparation: Ligate adapters and amplify IP'd material
Bioinformatic analysis: Perform read filtering and trimming, read specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc.

Need guidance?

Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.

Recommended products for ChIP-seq

Cat. No.	Product	Format
C01010171	Auto iDeal ChIP-seq kit for Histones This product must be used with the IP-Star Compact Automated System. Don’t risk wasting your precious sequencing samples. Diagenode’s validated&...	100 rxns
C01010172	Auto iDeal ChIP-seq Kit for Transcription Factors This product must be used with the IP-Star Compact Automated System. Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly sp...	100 rxns
C01010153	Auto Universal Plant ChIP-seq kit The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, ma...	24 rxns
B01080010	Bioruptor^® Pico sonication device The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing sam...	1 unit

C01019027 ChIP Cross-link Gold
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective in cr... 600 µl C01010051 iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit for Histones has everything you need for a succes... 4 chrom. prep./24 IPs C01010055 iDeal ChIP-seq kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly validated solution for robust transcription factor and other non-histone pro... 4 chrom. prep./24 IPs C01010132 True MicroChIP-seq Kit
Download the Manual The True MicroChIP-seq kit provides a robust ChIP protocol suitable for the investigation of histone modifications within chromatin from... 20 rxns C01010152 Universal Plant ChIP-seq kit
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize... 24 rxns C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker). 24 rxns C30010014 2 ml microtubes for SX-8G IP-Star^® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System. 100 pc C30080030 96 well microplates for IP-Star^®
Standard 96 well transparent plates made by polyestyrene and compatible with automated systems. Solid U-bottom plates free of DNAase and RNAase and human DNA. 10 pc C01011000 Auto ChIPmentation Kit for Histones - Replaced by C01011009
Contact us This product must be used with the IP-Star Compact Automated System. Diagenode’s latest technology for histone ChIP-seq, ... 24 rxns C01010058 Auto iDeal ChIP-seq Kit for Transcription Factors
This product must be used with the IP-Star Compact Automated System. Diagenode’s Auto iDeal ChIP-seq Kit for Transcription Factors is a highly sp... 24 rxns C03010010 Auto IPure kit v2
This product must be used with the IP-Star Compact Automated System. The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applicat... 100 rxns C02020011 Auto MethylCap kit
The Auto MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated... 48 rxns C15410337 BRD4 Antibody
Polyclonal antibody raised in rabbit against human BRD4 (Bromodomain Containing 4), using two KLH-conjugated synthetic peptides from the N-terminal and centr... 50 µg C01011010 ChIPmentation Kit for Histones - Replaced by C01011009
Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ... 24 rxns C15210021 H2A.Z Antibody
Monoclonal antibody raised in rabbit against histone variant H2A.Z, using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part o... 100 µg C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker). 24 rxns C03010015 IPure kit v2
Diagenode’s IPure kit is the only DNA purification kit using magnetic beads, that is specifically optimized for extracting DNA from&nb...; 100 rxns C17040004-50 Maize B73 inner stem ZmB1-UTR primer pair
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste... 50 µl C17040005-50 Maize B73 inner stem ZmCopia primer pair
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ... 50 µl C17040011-50 Poplar xylem PtrCopia-orth primer pair
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually... 50 µl C17040010-50 Poplar xylem PtrMYBTF1 primer pair
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p... 50 µl C17040009-50 Rice seedlings OsMADS6 primer pair
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly... 50 µl C15410368 SUV39H1 Antibody
Other names: KMT1A, MG44 Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic pept... 50 µg C17040006-50 Tomato leaves SlChr2-reg8 primer pair
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ... 50 µl C17040007-50 Tomato leaves SlChr4-NC1 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ... 50 µl